- illustrations of isolation and characterization of each cell type comprising normal and cancerous breast tissue: ->Please refer the above picture<-
- stroma specific genes, stromal component of breast tumours, Gene expression profiling, determination of stromal signatures, confirm, localize, observations of the expression of solitary fibrous tumour (SFT) and desmoid- type fibromatosis (DTF) specific genes, etc: gene microarray / tissue microarray (TMA)
-key genes associated with a specific disease (Roche???*!!!): cantilever-array (label free and amplification free)
-study of signalling in microenvironments, for manipulating the
behaviour of embryonic stem cells: synthesized biomaterial microarrays (Anderson et al, 2004) and ECM microarrays (Flaim et al, 2005) Additional platforms or small molecules (Bailey et al, 2004) in cell-based assays.
-proteomic profiling of the cancer microenvironment: antibody arrays
-study of micrometastasis: q-RT-PCR, as well as cellular imaging techniques, such as brighter and dual-fluorescence cell markers, inorganic labels that do not photo-bleach and longitudinal single cell imaging by MRI,
-circulating tumour cells (CTS): laser scanning cytometry with a multimarker real-time RT-PCR assay, immunomagnetic enrichment and fluorescent immunocytochemical characterization (an automated FDA approved system, CellSearch™, etc)
-study the cell fusion (HSCs and local stem-cell niche): Staining: staining cells expressing both the donor’s double marker (EGFP and beta-gal) and the recipients Y chromosome.
-reveal and analyse the cellular complexity: Living –cell micro array (by R&D Molecular cytomics),
-for stem-cell behaviour: labelling in animal studies: retroviral transduction with a market gene or labelling with thymidine or bromodeoxyuridine (BrdU)
-clinical detection of stem-cells: magnetic labelling and in vivo tracking of bone marrow cells by the use of magnetodentrimers or radioactive detection methods
-for assessment of time course of proliferation of stem-cells: generally using reporter gene: LecZ, by identification of galactosidase positive cells in tissue sections and the chromosome analysis by FISH (fluorescent insitu hybridisation),
-for separate different cell populations in cancer stroma expression: flow cytometry,
-to generate gene expression profiles of different components of the tumour microenvironment, including neoplastic and stromal cell types: SAGE (serial analysis of gene expression),
-epithelial–stromal interactions during mammary gland development: mouse model (paper to read: Review: Mouse models of breast cancer metastasis
By Anna Fantozzi and Gerhard Christofor). any other techniques?
-study of tumour microenvironment and drug resistance in hematologic malignancies (or study of environment mediated-drug resistance (EM-DR)): Stromal model using transwell (for reference: Tumour microenvironment and drug resistance in hematologic malignancies by Zhi-Wei Li etal. There is an illustration too)
- illustrations of isolation and characterization of each cell type comprising normal and cancerous breast tissue: ( here i will up-date the picture soon from library)
->Please refer the picture<-
-key genes associated with a specific disease (Roche???*!!!): cantilever-array (label free and amplification free)
-study of signalling in microenvironments, for manipulating the
behaviour of embryonic stem cells: synthesized biomaterial microarrays (Anderson et al, 2004) and ECM microarrays (Flaim et al, 2005) Additional platforms or small molecules (Bailey et al, 2004) in cell-based assays.
-proteomic profiling of the cancer microenvironment: antibody arrays
-study of micrometastasis: q-RT-PCR, as well as cellular imaging techniques, such as brighter and dual-fluorescence cell markers, inorganic labels that do not photo-bleach and longitudinal single cell imaging by MRI,
-circulating tumour cells (CTS): laser scanning cytometry with a multimarker real-time RT-PCR assay, immunomagnetic enrichment and fluorescent immunocytochemical characterization (an automated FDA approved system, CellSearch™, etc)
-study the cell fusion (HSCs and local stem-cell niche): Staining: staining cells expressing both the donor’s double marker (EGFP and beta-gal) and the recipients Y chromosome.
-reveal and analyse the cellular complexity: Living –cell micro array (by R&D Molecular cytomics),
-for stem-cell behaviour: labelling in animal studies: retroviral transduction with a market gene or labelling with thymidine or bromodeoxyuridine (BrdU)
-clinical detection of stem-cells: magnetic labelling and in vivo tracking of bone marrow cells by the use of magnetodentrimers or radioactive detection methods
-for assessment of time course of proliferation of stem-cells: generally using reporter gene: LecZ, by identification of galactosidase positive cells in tissue sections and the chromosome analysis by FISH (fluorescent insitu hybridisation),
-for separate different cell populations in cancer stroma expression: flow cytometry,
-to generate gene expression profiles of different components of the tumour microenvironment, including neoplastic and stromal cell types: SAGE (serial analysis of gene expression),
-epithelial–stromal interactions during mammary gland development: mouse model (paper to read: Review: Mouse models of breast cancer metastasis
By Anna Fantozzi and Gerhard Christofor). any other techniques?
-study of tumour microenvironment and drug resistance in hematologic malignancies (or study of environment mediated-drug resistance (EM-DR)): Stromal model using transwell (for reference: Tumour microenvironment and drug resistance in hematologic malignancies by Zhi-Wei Li etal. There is an illustration too)
- illustrations of isolation and characterization of each cell type comprising normal and cancerous breast tissue: ( here i will up-date the picture soon from library)
->Please refer the picture<-
No comments:
Post a Comment